Exp Taq 高 GC 聚合酶(dNTPs+)

产品货号:AG11507 / AG11508

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¥270¥970

产品描述
本制品是专门针对高 GC 含量模板设计的 PCR 扩增产品。DNA Polymerase、反应 buffer 都经过优化,非常适合于复杂、富含 GC 的 cDNA 和基因组 DNA 模板扩增,具有错配率低以及 高扩增性的特点。使用本制品扩增得到的大部分 PCR 产物 3’端带有一个 A 碱基,可直接克隆于 T 载体。
为了获得更好的扩增效果,本制品配有两种反应 Buffer:2X GC Reaction Buffer 与GC Enhancer Buffer;一般情况下建议首先使用 2X GC Reaction Buffer 进行扩增反应,如果不能得到理想的结果,可再向 PCR 反应体系中添加适量的 GC Enhancer Buffer 进行尝试。
产品组成
组分 AG11507 AG11508
Exp Taq DNA Polymerase(5 U / μl) 25 μl 100 μl
2X GC Reaction Buffer 1.25 ml 1 ml x 5 pc
GC Enhancer Buffer(5 M) 750 μl 1 ml x 3 pc
dNTP Mix(10 mM each) 100 μl 400 μl
保存与运输
保存温度:-20℃
运输温度:干冰或者 -20℃ 冰袋
XML 地图